Bioneer’s production capacity can accommodate orders ranging from milligrams to tens of grams of the purest DNA and RNA, including both standard and modified.  A large portion of our bulk oligo customers are involved in research requiring antisense oligos, which has opened the door to new approaches in the development of pharmaceuticals and target validation.  Some of the frequently used modifications are phosphorothioate bonds, 2'O-methyl RNA, and decoy oligos.

At Bioneer, large Scale Oligo synthesis refers to synthesis scales of 15µmol and above. 

For oligos at smaller synthesis scales, please refer to this page, or, for orders involving 96 oligos or more, please click here.

Frequently Used Modifications

The following modifications are most often requested for large scales oligos:

Phosphorothioates and Chimeric Oligos:
General oligos are subject to rapid degradation by nucleases.  Therefore, oligos for antisense application are usually synthesized with a phosphorothioate bond modification to make them resistant to nuclease activity.  In phosphorothioates, a sulfur atom replaces a binding oxygen in the oligo phosphate backbone.
2’O-Methyl RNA oligos:
2’O-Methyl RNA increases nuclease stability and affinity of the antisense oligo to the target RNA.
Decoy oligos:
Transfection of a cis-element double-stranded oligonucleotide, referred to as the Decoy, is a new and powerful class of antisense technology for gene therapy and transcriptional regulation.

For information on pricing & ordering please e-mail order.usa@bioneer.com.