Custom Oligo Plate File Upload Order
Oligo Type:
Plate
Ship Method:
Wet
Dry
Plate Arrangement:
A1, A2, A3
A1, B1, C1
Plate Type:
Deep Well
Shallow Well
Normalization:
Entire Yield
or
pmole
Normalization:
uM
and
uL
Plate Notes:
Scale:
25 nmole
50 nmole
200 nmole
1 umole
10 umole
15 umole
Purification:
Bio-RP
HPLC
PAGE
Upload File
Post Handling Services:
Custom plate($10.0)
Individual plate - Labeled($40.0)
Mixing primers($0.2)
Aliquoting to daughter Plates($0.2)
Concentration normalized - Custom($0.2)
Concentration normalized - Standard (100 pmol/ul)($0.15)
MALDI-TOF(SNP or Genotyping)($1.0)
File format:
Text(Tab Delimited)(*.txt)
<Plate Name> <Well Location> <Sequence Name> <Oligo Sequence> <Notes>
<Plate Name> <Well Location> <Sequence Name> <Oligo Sequence> <Notes>
<Plate Name> <Well Location> <Sequence Name> <Oligo Sequence> <Notes>
...
e.g.
Instructions:
√ For mixed bases, use the code letters as follows: R(A, g); Y(C, T); M(A, C); K(g, T); S(g, C); W(A, T); V(A, C, g); H(A, T, C); B(g, T, C); D(G, A, T); N(A, C, g, T)
√ Please note HPLC & PAGE is not available on the 25nmole scale
√ 25 nmole (max. length 60 bases) - 50 nmole (max. length 75 bases) - 200 nmole (max. length 110 bases) - 1.0 umole (max. length 130 bases)
10 umole (max. length 130 bases) - 15 umole (max. length 130 bases)
Valid Options:
Scale
Purification
5' Mods
Internal Mods
3' Mods
25 nmole
50 nmole
200 nmole
1 umole
10 umole
15 umole
Bio-RP
HPLC
PAGE
5C6AM
5PHOS
5BIOTEG
5THIOC6
5FAM
5TAMRA
5HEX
5TET
5C3SP
5C6SP
5C12SP
5C18SP
5AMC12
5METHDC
5DSSP
5IR700
5IR800
5BIOTIN
3C6AM
3PHOS
3BIOTEG
3FAM
3TAMRA
3C3SP
3C6SP
3C12SP
3DABCYL
3C18SP
3THIOC6
3DSSP
3BIOTIN
3METHDC
I
FL-DT
U
C18SP
*
C12SP
C6SP
AMC6DT
C3SP
DSSP
5BRODU
8OXODA
8OXODG
BIOTIN
METHDC