Custom Oligo Plate File Upload Order
Oligo Type:
Plate
Ship Method:
Wet
Dry
Plate Arrangement:
A1, A2, A3
A1, B1, C1
Plate Type:
Deep Well
Shallow Well
Normalization:
Entire Yield
or
pmole
Normalization:
uM
and
uL
Plate Notes:
Scale:
25 nmole
50 nmole
200 nmole
1 umole
10 umole
15 umole
Purification:
Bio-RP
HPLC
PAGE
Upload File
Post Handling Services:
Custom plate($10.0)
Individual plate - Labeled($40.0)
Mixing primers($0.2)
Aliquoting to daughter Plates($0.2)
Concentration normalized - Custom($0.2)
Concentration normalized - Standard (100 pmol/ul)($0.15)
MALDI-TOF(SNP or Genotyping)($1.0)
File format:
Text(Tab Delimited)(*.txt)
<Plate Name> <Well Location> <Sequence Name> <Oligo Sequence> <Notes>
<Plate Name> <Well Location> <Sequence Name> <Oligo Sequence> <Notes>
<Plate Name> <Well Location> <Sequence Name> <Oligo Sequence> <Notes>
...
e.g.
Instructions:
√ For mixed bases, use the code letters as follows: R(A, g); Y(C, T); M(A, C); K(g, T); S(g, C); W(A, T); V(A, C, g); H(A, T, C); B(g, T, C); D(G, A, T); N(A, C, g, T)
√ Please note HPLC & PAGE is not available on the 25nmole scale
√ 25 nmole (max. length 60 bases) - 50 nmole (max. length 75 bases) - 200 nmole (max. length 110 bases) - 1.0 umole (max. length 130 bases)
10 umole (max. length 130 bases) - 15 umole (max. length 130 bases)
Valid Options:
Scale
Purification
5' Mods
Internal Mods
3' Mods
25 nmole
50 nmole
200 nmole
1 umole
10 umole
15 umole
Bio-RP
HPLC
PAGE
5AMC6
5PHOS
5BIOTEG
5THIOC6
5METHDC
5FAM
5TAMRA
5HEX
5TET
5C3SP
5C6SP
5C12SP
5C18SP
5AMC12
3AMC6
3PHOS
3BIOTEG
3FAM
3TAMRA
3C3SP
3C6SP
3C12SP
3DABCYL
AMC6
BIOTEG
FAM
U
C18SP
*
3DABCYL
IC12SP