All oligos synthesized by Bioneer are purified using the BioRP purification system. This uses the oligo purification cartridge (OPC) technology and generates oligos that are 85% pure. This service is available free of cost on all oligos. We also offer HPLC and PAGE purification.

BioRP Purification
The BioRP cartridge functions on a basic trityl on selection. In addition to a de-salting, it removes most of the truncated products. This technology yields oligos that are superior in purity than that obtained by standard ethanol precipitation or gel filtration.

PAGE Purification
PAGE purification is recommended for oligos between 50-100 bases. This yields oligos with purity levels over 95% purity. Subsequently yield obtained is lower than other purification techniques.

HPLC Purification
HPLC is recommended for both modified and unmodified oligos up to 50 bases in length. Our HPLC purifications always start with a trityl on purification, and are often followed by a second full gradient trityl off HPLC. This is our Double RP-HPLC Purification process that gives an extra level of purification. In some cases, the initial trityl-on purification is entirely sufficient and a cartridge is utilized in the subsequent trityl-off oligo processing. This RP-HPLC purification process can yield highly pure material, especially when the oligonucleotide is not complex. When synthesizing compounds that require post-synthetic modification, such as dye labeling, we will perform an additional RP-HPLC purification.